FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37751703
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC229300.024.66650413
GGGATTA260800.024.10298212
GATTACA245800.022.76829714
ACGTCGA35750.022.2260111
ATTACAG273100.020.13356815
TGGGATT379100.018.62190611
TTACAGG308150.017.59360916
AAAGTGC280950.016.2206544
TACAGGC270900.016.1629417
CGTCGAG54450.015.6847342
CCAAAGT324600.015.0853192
CTGGGAT508950.014.17346610
AAGTGCT329750.013.9581895
CAAAGTG347250.013.6578493
ACAGGCG195750.013.46374218
ACGCGAG54700.013.18107323
GTCGAGA63100.013.0908523
CCCAAAG459300.012.8338611
CACGCGA45100.012.80496122
GCTAATT115250.012.26894741
Produced by FastQC (version 0.11.5)