FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17317421
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA187600.025.13095512
GGATTAC173600.024.63723413
ACGTCGA16950.024.1610321
GATTACA185650.022.99993314
CGTCGAG33300.022.7043381
TGGGATT231400.021.720411
ATTACAG200800.020.8813515
TTACAGG209850.019.66387416
TACAGGC184300.019.12365317
GTCGAGA39450.018.986442
AAAGTGC172650.018.1239974
CTGGGAT282550.017.86283710
CGGCTAA25650.017.1929339
CCAAAGT199800.016.9748132
ACCGCGC49950.016.60669931
ACAGGCG117900.016.53514918
AAGTGCT195950.016.2547535
CGTGAGC96200.016.19008623
CAAAGTG204800.015.9110563
GCGTGAG96950.015.84823622
Produced by FastQC (version 0.11.5)