FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17317421
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC184050.025.97613
GGGATTA200400.025.84752512
GATTACA193950.024.65022514
TGGGATT246100.022.46940611
ATTACAG209350.022.4691315
TTACAGG219450.020.98874716
AAAGTGC178800.020.2381294
TACAGGC192200.020.15831217
CTGGGAT285950.019.20343810
ACAGGCG122500.018.74267218
CCAAAGT202700.018.0785732
GGCGTGA103050.017.76319921
AAGTGCT205500.017.625835
CAAAGTG209850.017.5943033
CGTGAGC104850.017.59162323
AGGCGTG116000.017.37928620
GCGTGAG105550.017.07719222
CAGGCGT116000.016.77583919
ACCGCGC52700.016.4703331
CCCAAAG256150.015.8837711
Produced by FastQC (version 0.11.5)