FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31468870
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC316550.023.9930713
GGGATTA345300.023.78962912
GATTACA337250.022.70681414
ATTACAG361000.020.85458815
TGGGATT425950.020.65753711
ACGTCGA28300.020.5323721
TTACAGG367800.019.78386716
TACAGGC340450.018.64892417
AAAGTGC309300.017.8802074
CTGGGAT514350.017.15500510
CGAGTAG74300.016.6765023
CCAAAGT355800.016.5661582
AGTAGCT243350.016.469115
CGGCTAA52000.016.28837239
AAGTGCT350900.016.119595
ACAGGCG208150.016.09182518
CAAAGTG375300.015.6029933
CCGAGTA85050.015.3915722
GCTAATT199450.015.17916141
CGTCGAG41100.015.0736052
Produced by FastQC (version 0.11.5)