FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31468870
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA354000.023.72825212
GGATTAC330500.023.63635613
GATTACA343200.022.88429314
ATTACAG362100.021.40952915
TGGGATT429850.020.69700211
TTACAGG378350.019.80552116
TACAGGC342400.018.21522317
AAAGTGC314350.018.1464884
CTGGGAT526050.017.07839210
CCAAAGT361700.016.9051932
AAGTGCT350550.016.392475
ACAGGCG214500.016.12101218
CAAAGTG372350.015.9499493
AGTAGCT231300.015.40257555
GCGTGAG181500.015.07986822
CGGCTAA50650.014.92601339
ATCGCTT58050.014.89257427
CCCAAAG473400.014.7204871
GGCGTGA185250.014.66124921
CGTGAGC188950.014.50377223
Produced by FastQC (version 0.11.5)