FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35979620
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA34150.025.522831
GGGATTA232250.023.0727112
GGATTAC213600.022.77664213
GATTACA239000.020.44338414
ATTACAG274950.017.9232715
CGTCGAG52150.017.2489072
TGGGATT341050.017.23114811
TTACAGG296300.016.46640616
TACAGGC260150.015.91580617
AAAGTGC275300.014.3795274
CTGGGAT443300.013.65160410
ACAGGCG174050.013.63400318
GTCGAGA65300.013.5609873
CCAAAGT325200.013.2599812
CGAGTAG57250.012.6554863
AGTAGCT179050.012.5306715
CAAAGTG345450.012.4624643
AAGTGCT325750.012.3352535
TGATCAA181000.012.0281669
CCCAAAG440800.011.7845361
Produced by FastQC (version 0.11.5)