FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35979620
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA237050.022.26477412
GGATTAC217850.022.23497213
GATTACA239100.020.18590514
ATTACAG277100.017.2661515
TGGGATT338950.016.98551411
TTACAGG296400.015.82322316
TACAGGC259300.015.72479817
AAAGTGC281850.014.1919754
CCAAAGT326600.013.7065122
CTGGGAT441700.013.28783110
ACAGGCG178000.012.839807518
CAAAGTG339150.012.66126253
AAGTGCT327600.012.4451895
GGCGTGA157550.012.12949221
CCCAAAG440350.012.0472091
GCGTGAG161750.011.68472422
CGTGAGC167600.011.44382823
AGGCGTG174850.011.3497220
ACCGCGC71950.011.33409431
CTACTCG50350.011.261292
Produced by FastQC (version 0.11.5)