FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24808917
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC166750.026.65586713
GGGATTA193050.025.58068512
GATTACA176900.024.71105614
ATTACAG201800.021.07229215
TGGGATT255650.020.37098711
TTACAGG215350.019.14507716
AAAGTGC192450.018.4398164
TACAGGC187000.017.94872917
CCAAAGT229700.016.644532
AAGTGCT225150.015.9947465
CAAAGTG233650.015.8193933
CTGGGAT331550.015.64400510
ACAGGCG132550.014.65449218
CCCAAAG313000.014.3952511
CGTGAGC122850.013.93142923
GCGTGAG123450.013.92042122
GGCGTGA121850.013.90214421
ACCGCGC59100.013.50275731
AGGCGTG132700.013.16081620
CAGGCGT126400.013.124577519
Produced by FastQC (version 0.11.5)