FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29685436
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA226050.024.10739712
GGATTAC212950.024.0453713
GATTACA234550.022.06979214
ACGTCGA26500.021.1355111
ATTACAG262200.019.59564615
TGGGATT315400.018.9426611
TTACAGG274200.018.62315816
TACAGGC243100.018.44298217
AAAGTGC251400.015.8305154
ACAGGCG166400.015.4807318
CTGGGAT404400.015.16326810
CCAAAGT283900.015.1030792
CGTCGAG42600.014.873311
ACCGCGC70150.014.6184931
GGCGTGA146400.014.58332221
GCGTGAG147700.014.50238222
CGAGTAG50200.014.3635783
CGGCTAA37000.013.99982439
CAAAGTG299550.013.92855553
CGTGAGC152800.013.92669123
Produced by FastQC (version 0.11.5)