FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29685436
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC237050.024.730313
GGGATTA254750.024.56433112
GATTACA255100.023.2139814
ATTACAG285050.020.73806815
TGGGATT339000.019.8841711
TACAGGC258900.019.39900817
TTACAGG300650.019.14980516
AAAGTGC266700.017.2031764
ACAGGCG173900.016.48329418
CCAAAGT312900.016.147512
CTGGGAT426200.015.96335710
ACCGCGC69550.015.49973431
GGCGTGA156550.015.31427621
CAAAGTG325600.015.1679853
AAGTGCT303750.015.0816955
GCGTGAG163250.014.7286622
AGGCGTG172500.014.648746520
CAGGCGT171200.014.3102819
CGTGAGC168800.014.30659723
CCCAAAG409850.014.2010741
Produced by FastQC (version 0.11.5)