FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22539893
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC183750.023.84725613
GGGATTA200700.023.24624612
GATTACA193900.022.52678114
ATTACAG219250.019.81050315
TGGGATT258850.019.45756511
TTACAGG233350.018.1185116
TACAGGC203600.017.75766617
AAAGTGC203000.017.7253634
ACGTCGA18700.017.5958521
CGTCGAG32200.017.3935471
CCAAAGT229550.016.3762842
ACAGGCG139500.016.08226418
CTGGGAT330600.015.49940610
ACCGCGC59250.015.47659131
GGCGTGA123400.015.45772721
CAAAGTG239400.015.4250073
AAGTGCT234550.015.4159275
GCGTGAG126600.015.26053122
CGTGAGC128400.015.04663423
AGGCGTG134650.014.73801720
Produced by FastQC (version 0.11.5)