FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19474717
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC163600.026.16366813
GGGATTA184550.025.92440612
GATTACA177100.024.09021414
ATTACAG193900.021.75027315
TGGGATT242350.020.78127511
TTACAGG204550.020.19007716
TACAGGC175800.019.60978717
AAAGTGC176900.018.9926454
CCAAAGT203800.017.9538352
ACAGGCG114850.017.36984818
AAGTGCT200750.016.9453965
CTGGGAT302600.016.88627610
CAAAGTG213700.016.69033
CGGCTAA25500.016.19573639
CGTGAGC104550.016.03500723
GCGTGAG104500.015.90871322
GGCGTGA107000.015.86406821
CCCAAAG273150.015.5482031
AGGCGTG112700.015.40308420
CAGGCGT111850.015.11339919
Produced by FastQC (version 0.11.5)