FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26352090
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA30550.024.17741
GGGATTA269350.023.41568812
GGATTAC258050.022.88110713
GATTACA265100.022.37827514
ATTACAG280100.020.94245315
TGGGATT327700.020.44252811
TTACAGG298650.019.53621916
TACAGGC280450.018.37048717
CGTCGAG53400.018.2239231
AAAGTGC266500.018.0201724
TGATCAA146200.017.6200439
CCAAAGT293650.016.7113932
GTCGAGA58550.016.499732
CTGGGAT417850.016.19975510
GACTTGT160200.016.0810383
AAGTGCT306000.015.9573145
TTGTGAT163600.015.918426
ACAGGCG216000.015.63658218
CAAAGTG313050.015.5305663
ACTTGTG172750.015.3789194
Produced by FastQC (version 0.11.5)