FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26352090
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG302580.11482201221990362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC235550.021.48523313
GGGATTA250350.021.36132212
GATTACA244200.020.73871614
ATTACAG258150.019.04860515
TGGGATT306850.018.87647811
TTACAGG273700.017.77456516
TACAGGC254200.016.63222517
AAAGTGC256750.015.4028044
CTGGGAT399900.014.87790110
CCAAAGT289100.014.582112
ACAGGCG190650.014.20901518
ACCGCGC88900.013.58288431
CAAAGTG297900.013.5588343
AAGTGCT296000.013.5375935
GGCGTGA170150.013.4731521
GCGTGAG177950.012.92192422
CGTGAGC182750.012.83149423
CCCAAAG379400.012.7445811
GCTAATT103300.012.65181
CAGGCGT183500.012.64539219
Produced by FastQC (version 0.11.5)