FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27889088
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA319050.022.97052212
GGATTAC300150.022.53969413
GATTACA323150.021.19552814
TGGGATT388550.019.76253311
ATTACAG346650.019.64758915
TTACAGG361150.018.38391916
TACAGGC316600.017.91969117
AAAGTGC314000.017.4985734
ACCGCGC84400.017.45895631
ACAGGCG204350.016.69891718
GGCGTGA184500.016.50390421
CTGGGAT463850.016.47847710
CGTGAGC191650.016.2533723
GCGTGAG189200.016.05689822
CCAAAGT349900.015.8908222
AAGTGCT350450.015.8381175
AGGCGTG197900.015.598320
CAAAGTG359050.015.4414833
CAGGCGT194300.015.29285519
CACCGCG105450.014.43857730
Produced by FastQC (version 0.11.5)