FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28214864
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG320910.11373792196907276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC271800.021.63294613
GGGATTA288100.021.52645312
GATTACA294700.020.03527814
ATTACAG320100.018.81722815
TGGGATT363900.017.9561311
TACAGGC292350.017.71824317
TTACAGG333300.017.5365116
ACAGGCG192850.015.86176418
AGTAGCT163750.015.1314345
AAAGTGC305450.015.0552774
CGAGTAG49150.015.0261023
CTGGGAT427500.015.02262310
CGGCTAA36650.014.9929739
GCGTGAG171650.014.66052122
GGCGTGA169650.014.60641721
CTACTCG41800.014.5748582
CGTGAGC174150.014.36967423
ACCGCGC74050.014.3215631
CCAAAGT336200.014.2052222
AGGCGTG183750.014.11396420
Produced by FastQC (version 0.11.5)