FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26914688
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG269950.10029839469066108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA225950.021.39121812
GGATTAC208100.021.34242213
GATTACA214500.020.82015814
ATTACAG232400.018.77979915
TGGGATT291200.017.93212111
TTACAGG248900.017.3942716
TACAGGC238300.015.84743117
AAAGTGC234850.014.4849214
CCAAAGT268150.013.82762052
CTGGGAT389450.013.72245510
ACAGGCG171450.013.69761218
GGCGTGA153800.013.15329521
CAAAGTG281200.013.044773
GCTAATT102800.012.986431
AAGTGCT269300.012.9826435
CGGCTAA30650.012.56114739
GCGTGAG165650.012.38138522
CGTGAGC166000.012.31311223
AGGCGTG174400.012.00076420
AGTAGCT138950.011.938615
Produced by FastQC (version 0.11.5)