FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27130107
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC321300.024.31351313
GGGATTA341400.024.2148312
GATTACA336100.023.82599314
ATTACAG357850.022.34859715
TGGGATT396250.022.04694411
TACAGGC331000.020.78845417
TTACAGG380000.020.69587316
AAAGTGC332750.020.0286454
ACGTCGA26150.019.544181
ACAGGCG237650.019.46974818
ACCGCGC106250.019.3032931
GGCGTGA215100.018.77739121
CCAAAGT368400.018.641142
AATCGCT48500.018.40207326
CGTCGAG49400.018.2822271
AAGTGCT367850.018.2416655
CTGGGAT483700.018.16973110
CGTGAGC225850.018.06949223
CAAAGTG379550.017.9646133
CAGGCGT231050.017.87476319
Produced by FastQC (version 0.11.5)