FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27130107
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC272400.022.47196213
GGGATTA289800.022.42690712
GATTACA284500.021.81149914
ATTACAG305950.020.05351615
TGGGATT351750.019.70094111
TTACAGG321600.018.729416
TACAGGC285800.017.7078217
AAAGTGC295600.016.8593734
CTGGGAT436500.016.01182610
ACAGGCG205550.015.9373118
ACCGCGC92850.015.86979331
GGCGTGA184400.015.84837121
CCAAAGT328000.015.8424252
AAGTGCT327200.015.4448945
GCGTGAG191100.015.42092622
CAAAGTG337150.015.0950553
CGTGAGC198300.014.94917923
AGGCGTG204200.014.8428220
CAGGCGT200650.014.52984219
CCCAAAG425650.013.7803431
Produced by FastQC (version 0.11.5)