FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12875207
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA11150.027.0001771
GGATTAC96800.025.77960413
GGGATTA110600.024.58836612
GATTACA103650.023.53561414
ATTACAG113650.021.12605715
TGGGATT147650.020.36285411
TTACAGG120100.019.729216
TACAGGC105400.018.79517417
AAAGTGC107050.017.6888524
CGTCGAG19100.017.2281061
ATCGCTT18150.016.7768927
CGAGTAG24550.015.9681853
CTGGGAT185100.015.95934110
ACAGGCG72650.015.85005518
CCAAAGT123700.015.7605432
AATCGCT15300.015.32676626
GCGTGAG61200.015.15519822
AAGTGCT125150.015.1311045
CCGAGTA29000.014.8454312
GTCGAGA22700.014.6481712
Produced by FastQC (version 0.11.5)