FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875758
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35030.18675116939391967No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA400.002420874834.9968958
GATTACG552.9947952E-431.81705314
ACGATAA604.987292E-429.16485669
GACGTAT1001.39976E-524.49913251
TCGATTC901.9299683E-423.33250859
TAACGAT750.001833838223.33250867
ACTTATC800.002665840621.8777263
CGACATA800.002667078721.87597526
TATCGGT1003.9358498E-420.99925634
AACGATA850.00379300920.58695668
ATTGCGA900.00527428519.4468673
CTAACGA900.005279164819.44375666
TGCGTTA900.005279164819.44375611
CTAATAT900.005279978719.44323770
GGATTAC16500.019.3023513
GATTACA17500.018.99932914
TATACTC1301.05859384E-418.84498470
GGGATTA16650.018.28764212
AATCAAC1150.001008580918.26022361
ATTACAG18200.017.6916815
Produced by FastQC (version 0.11.5)