FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24856297
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA41950.044.3926621
CGTCGAG79650.027.9513551
GTCGAGA107450.020.7828312
GGATTAC219550.019.33687813
GGGATTA233800.019.2811912
GATTACA237550.018.2253314
TCGAGAC128950.017.7520893
ATTACAG268050.016.34748815
TGGGATT298950.016.26213311
TACAGGC237200.015.92108717
TTACAGG276500.015.44284216
CGAGACT192100.015.3614331
TGATCAA255550.015.2987679
GACTTGT260100.014.9912533
ACTTGTG272700.014.6195594
GTGATCA275200.014.3212348
GATCAAG273150.014.31292610
AAAGTGC243350.014.1102064
TTGTGAT285600.013.9597276
GAGACTT288050.013.8902441
Produced by FastQC (version 0.11.5)