FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24856297
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG353500.1422174831592976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA260100.020.89710612
GGATTAC244450.020.84621613
GATTACA265500.019.37802914
ATTACAG289650.017.7744415
TGGGATT328050.017.45413411
TACAGGC258100.016.8826217
TTACAGG296150.016.74621616
ACAGGCG171250.015.45075718
AAAGTGC269950.014.9998134
ACCGCGC69700.014.81357931
CGAGTAG44300.014.6958853
CTGGGAT388350.014.47341910
CCAAAGT303350.014.1972152
GGCGTGA155900.014.18841521
AGTAGCT146800.013.9463725
GCGTGAG157600.013.83549722
CGTGAGC161300.013.67001723
AAGTGCT301450.013.5135415
CAGGCGT167050.013.42976319
AGGCGTG170550.013.40044220
Produced by FastQC (version 0.11.5)