FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25730800
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC297050.024.3779613
GGGATTA319900.024.16835412
GATTACA316300.022.9607114
ACGTCGA24450.022.9076291
TGGGATT386050.021.35993211
ATTACAG349750.020.57466115
TACAGGC306600.020.2510317
TTACAGG357800.019.71058516
ACCGCGC86300.019.38556731
ACAGGCG199250.019.1993918
AAAGTGC306900.018.8189644
CGTGAGC178000.018.54199623
CTGGGAT446050.018.29843710
GCGTGAG177600.018.2881522
GGCGTGA178000.018.18799421
CAGGCGT185350.017.99547819
AGGCGTG187500.017.97572520
AAGTGCT333750.017.6617835
CCAAAGT337700.017.402832
CAAAGTG352300.016.552753
Produced by FastQC (version 0.11.5)