FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16136764
Sequences flagged as poor quality0
Sequence length110
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1818941.1272024552134492No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG834980.5174395560349027No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT312250.193502241217632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG341250.067.594342
GTATCAA348550.066.722079
GGTATCA355250.065.197838
AAGCAGT356650.064.977981
TGGTATC359600.064.331477
GTGGTAT378150.061.268916
AGTGGTA391950.059.1353155
CAGTGGT396100.058.51234
GCAGTGG522150.044.5050283
ATCAACG329400.038.42043310-11
CAACGCA350850.036.13353712-13
ACGCAGA356650.035.56579214-15
AGAGTAC362150.034.89014418-19
TCAACGC339950.034.21757512-13
TATCAAC364600.032.12993210-11
AACGCAG364300.032.00658814-15
GCAGAGT396900.031.91376516-17
CGCAGAG380300.030.62638516-17
GTACTTT380250.030.28298422-23
AGTACTT426600.029.45886620-21
Produced by FastQC (version 0.11.5)