FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14586232
Sequences flagged as poor quality0
Sequence length110
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1165920.7993291207763595No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG618680.4241534071307792No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT176750.12117591438282346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA244150.066.1229
GGTATCA250250.064.401728
TGGTATC258050.062.3483437
AGCAGTG265200.060.5471532
AAGCAGT271300.059.319871
GTGGTAT275400.058.773646
AGTGGTA281150.057.270125
CAGTGGT292450.055.0366444
GCAGTGG403600.040.0316663
ATCAACG226650.038.81702410-11
CAACGCA240100.036.73309312-13
ACGCAGA249750.035.2948914-15
TCAACGC229700.035.27201512-13
AGAGTAC254050.034.37002618-19
AACGCAG255250.031.87553414-15
TATCAAC263700.030.94773110-11
GCAGAGT295350.029.78443516-17
GTACTTT274100.029.23230222-23
CGCAGAG278200.029.1716516-17
AGTACTT316350.027.46007520-21
Produced by FastQC (version 0.11.5)