FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15695204
Sequences flagged as poor quality0
Sequence length110
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1375220.8762039665110438No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG610070.38869835651706086No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT217640.13866656336547137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA282250.066.801599
GGTATCA290250.064.651168
TGGTATC296150.063.3347057
AGCAGTG299500.062.1891062
AAGCAGT304400.061.4200251
GTGGTAT318800.058.8600166
CAGTGGT333950.056.0924764
AGTGGTA335300.055.8459745
GCAGTGG458000.041.3038523
ATCAACG268750.038.2845210-11
CAACGCA290400.035.54527312-13
ACGCAGA296600.034.713914-15
AGAGTAC298700.034.37566418-19
TCAACGC278200.033.88899212-13
TATCAAC304550.031.30027610-11
AACGCAG302450.031.16188614-15
GCAGAGT334950.030.76386816-17
CGCAGAG324050.029.05380416-17
GTACTTT323200.028.8344522-23
AGTACTT365950.027.9141420-21
Produced by FastQC (version 0.11.5)