FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13175636
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC51200.017.9446981
ATGAATG51000.017.62180910
GCGCGAT30700.017.3897571
CAATGAA48850.017.3122528
TGCTACC49650.017.2564811
CAATGGA53700.017.2379048
TTGACTT60100.017.1670571
TTGTAGG39150.017.1604271
GACCTGG39300.017.145811
TGCGTGG49400.017.1211931
TGAGCCG37750.017.0817891
GGGACCT37900.017.0141851
TGCCAAA43000.016.9492191
GAAATGA58250.016.8712861
GGCTCGA33850.016.864291
GGAAAGC45900.016.85851
TATGCCC46200.016.8355871
ATGCGAT32650.016.8104671
CGGACAC47200.016.7754361
CGTTGGG45750.016.71711
Produced by FastQC (version 0.11.5)