FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13175636
Sequences flagged as poor quality0
Sequence length110
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3336682.5324621900605027No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG719250.5458939515329658No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT597090.45317736464486424No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT254990.19353145457266732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT518150.087.419541
AGCAGTG564200.079.977772
GTATCAA645400.070.8620769
TGGTATC644550.070.654397
GGTATCA646600.070.489878
GTGGTAT655000.069.680586
CAGTGGT661950.068.207584
AGTGGTA668100.067.727075
GCAGTGG732650.061.646723
ATCAACG631350.039.0484310-11
CAACGCA642750.038.44967312-13
ACGCAGA659100.037.41106414-15
AGAGTAC657650.037.3281118-19
TCAACGC637200.036.0095812-13
GCAGAGT688600.035.8010716-17
AACGCAG658550.034.79662714-15
TATCAAC665350.034.5677110-11
AGTACTT710950.034.38924820-21
CGCAGAG674250.033.94421416-17
GTACTTT682000.033.24775322-23
Produced by FastQC (version 0.11.5)