FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15236198
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC61800.018.2635631
GCCTCTA100350.017.6832071
GCGCGAT47800.017.6101971
CCGGGAT38700.017.2303831
GAATGAA43500.017.1906531
GACCTGG56700.017.1381231
GGGAATG45550.016.9656771
ACTGATG38900.016.9618951
CGGACTG34900.016.929421
GGAACTT50550.016.9092751
ATGCGAT43150.016.8667181
TTGACTT67950.016.8312591
ATTGGAC57450.016.6011581
CCCTCCT70700.016.5725081
ACGAGGA53700.016.5503621
ATCGAGT37950.016.4908331
AGAATGT59550.016.4846558
CTCTACG57700.016.4772511
TGCTACC46000.016.4737111
TATTACC58000.016.4437331
Produced by FastQC (version 0.11.5)