FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15236198
Sequences flagged as poor quality0
Sequence length110
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1345200.8828974262476768No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG713330.4681811039735766No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT211630.1388994813535503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA273600.064.9752969
GGTATCA277350.063.9558758
TGGTATC281950.063.0116847
AGCAGTG285600.061.952052
AAGCAGT290850.061.0448461
GTGGTAT299200.059.559166
AGTGGTA318200.055.775125
CAGTGGT321450.055.0723274
GCAGTGG434300.041.1869163
ATCAACG255050.038.1360310-11
CAACGCA270000.036.19294412-13
TCAACGC259000.034.45707312-13
ACGCAGA283500.034.41613414-15
AGAGTAC282400.034.3485118-19
AACGCAG286250.031.17842114-15
TATCAAC290500.030.81512810-11
GCAGAGT320550.030.6175116-17
GTACTTT305350.028.92610722-23
CGCAGAG312000.028.70597316-17
AGTACTT350200.027.6675820-21
Produced by FastQC (version 0.11.5)