FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14477627
Sequences flagged as poor quality0
Sequence length110
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1130290.7807149610913446No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG714560.4935615484498944No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT176200.12170502804085227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA237600.065.900239
GGTATCA244150.063.9357768
TGGTATC251850.061.9926577
AGCAGTG254750.061.3257372
AAGCAGT270400.058.090921
GTGGTAT269850.057.9452066
AGTGGTA282000.055.4231155
CAGTGGT282300.055.369234
GCAGTGG396250.039.8737533
ATCAACG218700.038.60872310-11
CAACGCA232600.036.4529712-13
TCAACGC221800.035.34415412-13
ACGCAGA242400.034.83590714-15
AGAGTAC244150.034.4382818-19
AACGCAG248400.031.70163714-15
TATCAAC257150.030.90488410-11
CGCAGAG263650.029.82953316-17
GCAGAGT285100.029.74719616-17
GTACTTT266550.028.9237822-23
AGTACTT302000.027.77963620-21
Produced by FastQC (version 0.11.5)