FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15789617
Sequences flagged as poor quality0
Sequence length110
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1513940.9588199637774621No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG681900.4318660800955464No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT259390.16427884222904204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA310350.065.4278269
AGCAGTG311850.064.690392
GGTATCA318000.063.60348
AAGCAGT325850.062.109961
TGGTATC325950.061.99627
GTGGTAT344100.058.8391386
AGTGGTA352850.057.1807825
CAGTGGT359450.056.3198554
GCAGTGG484400.042.2068983
ATCAACG291750.038.17085610-11
CAACGCA312000.035.7580912-13
ACGCAGA317300.035.0388214-15
AGAGTAC324500.034.26354618-19
TCAACGC300700.033.8248512-13
AACGCAG323050.031.54923814-15
GCAGAGT355500.031.35620716-17
TATCAAC329500.031.15480610-11
GTACTTT345050.029.366222-23
CGCAGAG347050.029.33936116-17
AGTACTT387450.028.62028120-21
Produced by FastQC (version 0.11.5)