FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16016390
Sequences flagged as poor quality0
Sequence length110
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1637771.0225587663637061No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG741650.4630569060818325No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT262870.16412562381410542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA314650.067.38179
GGTATCA324250.065.176478
AAGCAGT329600.064.440121
AGCAGTG329000.064.3442
TGGTATC331500.063.779227
GTGGTAT353700.060.0211076
CAGTGGT364450.058.2009934
AGTGGTA369450.057.4733665
GCAGTGG497150.043.0619743
ATCAACG299400.038.5956410-11
CAACGCA321550.035.949112-13
ACGCAGA329800.035.1410514-15
AGAGTAC332550.034.647818-19
TCAACGC310250.034.22480812-13
TATCAAC334300.031.9534710-11
AACGCAG333400.031.90704714-15
GCAGAGT367650.031.55216616-17
GTACTTT353800.029.60819822-23
CGCAGAG360200.029.57697916-17
AGTACTT399350.028.77293820-21
Produced by FastQC (version 0.11.5)