FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14614090
Sequences flagged as poor quality0
Sequence length110
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7685095.258685282491076No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1070220.7323206576666764No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT443030.3031526424156414No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA274420.18777768578132473No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT261340.1788274192919299No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTAA159640.10923704452347016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1100400.095.543471
AGCAGTG1158900.090.592312
GTATCAA1180900.089.6429149
TGGTATC1179150.089.604057
GGTATCA1182650.089.3506858
GTGGTAT1196600.088.502226
AGTGGTA1225550.085.714945
CAGTGGT1225150.085.678544
GCAGTGG1301700.080.696223
ATCAACG1170150.046.72606710-11
CAACGCA1175450.046.54958312-13
ACGCAGA1198100.045.66129714-15
AGAGTAC1198100.045.61788618-19
TCAACGC1170050.045.35539612-13
GCAGAGT1224000.044.70413216-17
TATCAAC1196400.044.45348710-11
AACGCAG1200300.044.27767614-15
CGCAGAG1212850.043.75142316-17
GTACTTT1214550.043.38232422-23
GAGTACT1216050.043.35347720-21
Produced by FastQC (version 0.11.5)