FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15329951
Sequences flagged as poor quality0
Sequence length110
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1337860.8727098997250545No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG741280.483550143115265No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT211260.13780865966238248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA268750.065.678729
GGTATCA274600.064.030468
AGCAGTG281050.062.744812
TGGTATC281250.062.5863467
AAGCAGT290700.060.9380761
GTGGTAT299150.059.1159936
CAGTGGT310750.056.734014
AGTGGTA319300.055.2846265
GCAGTGG429850.041.436653
ATCAACG251900.037.71048710-11
CAACGCA263850.036.11458212-13
TCAACGC253600.034.81642512-13
AGAGTAC275550.034.35147518-19
ACGCAGA278300.034.22593714-15
AACGCAG285100.031.10203414-15
TATCAAC288050.030.94720510-11
GCAGAGT318800.029.98473416-17
GTACTTT302100.028.95130722-23
CGCAGAG305750.028.94266316-17
GAGTACT314200.027.93109120-21
Produced by FastQC (version 0.11.5)