FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14908019
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGGTCTTGATTAATGAAAACATTC338040.2267504488691623No Hit
GTCTCGTTCGTTATCGGAATTAACCA200030.13417611018606831No Hit
CGTTCGTTATCGGAATTAACCAGACA175280.11757430682104711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTACG1006200.019.7968251
GGACAGA378450.019.65671
CTACGGT976750.019.647763
TACGGTA976550.019.633354
ACGGTAG977250.019.6224235
CGGTAGC978550.019.6148176
GGTAGCC979150.019.6123927
TACTTGT518850.019.606521
GGACGTC268150.019.6064471
AGCCTCG975550.019.60329210
TCTACGG981800.019.5937542
GTAGCCT980650.019.5825238
ACTTGTG508000.019.5671462
CTAACTT443300.019.5272241
TAGCCTC982500.019.516539
TTGTGTC507050.019.5109064
CTTGTGT509300.019.450243
CTTTCAA319700.019.4328862
TAGATGT320950.019.42879510
TTTCAAG317150.019.4186533
Produced by FastQC (version 0.11.5)