FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14908019
Sequences flagged as poor quality0
Sequence length110
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7576905.082432481471884No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1105660.7416545417603775No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT444600.2982287586298354No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT260410.17467780259738064No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA256660.1721623778451047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1078850.094.763671
AGCAGTG1137700.089.668532
GTATCAA1154550.089.0890359
TGGTATC1153900.088.875577
GGTATCA1157200.088.659228
GTGGTAT1167700.087.867136
AGTGGTA1200100.084.977215
CAGTGGT1206200.084.475114
GCAGTGG1278600.079.722143
ATCAACG1144550.046.63844310-11
CAACGCA1149650.046.508212-13
ACGCAGA1172850.045.5310514-15
AGAGTAC1172550.045.4260718-19
TCAACGC1144200.045.16184612-13
GCAGAGT1195600.044.63280516-17
TATCAAC1175250.044.0309810-11
AACGCAG1175250.044.01093714-15
CGCAGAG1190250.043.3193816-17
GAGTACT1186900.043.14560320-21
AGTACTT1227700.043.08421320-21
Produced by FastQC (version 0.11.5)