FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14752097
Sequences flagged as poor quality0
Sequence length110
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1376030.9327690836089269No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG869760.5895839757561248No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT214240.14522681080527058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA269500.066.7099
GGTATCA273450.065.571838
AGCAGTG281150.063.743012
TGGTATC281950.063.462947
AAGCAGT295600.060.9668851
GTGGTAT299100.059.9079136
AGTGGTA305950.058.4117245
CAGTGGT311400.057.7030684
GCAGTGG422250.042.922423
ATCAACG250650.038.85186810-11
CAACGCA263650.036.88131712-13
TCAACGC253950.035.45413612-13
ACGCAGA276000.035.31134414-15
AGAGTAC278500.034.95802718-19
AACGCAG281200.032.2171114-15
TATCAAC285950.031.83741810-11
CGCAGAG296850.030.39680716-17
GCAGAGT322050.030.38410616-17
GTACTTT298550.029.98356622-23
AGTACTT335150.029.01699420-21
Produced by FastQC (version 0.11.5)