FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16712585
Sequences flagged as poor quality0
Sequence length110
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1484170.8880553187911984No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG639930.38290306376901No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT240750.14405311925115116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA309150.065.827359
GGTATCA314850.064.285558
AGCAGTG321050.063.111032
TGGTATC323400.062.4831627
AAGCAGT328850.061.6756481
GTGGTAT339150.059.7432866
AGTGGTA348250.058.010855
CAGTGGT361700.055.967134
GCAGTGG506950.040.3394783
ATCAACG289450.037.99473610-11
CAACGCA307950.035.71563712-13
ACGCAGA317700.034.63809614-15
TCAACGC296200.034.45504812-13
AGAGTAC319250.034.4448718-19
AACGCAG323000.031.70288314-15
TATCAAC328850.031.1339710-11
GCAGAGT372850.029.75164216-17
GTACTTT344200.029.563722-23
CGCAGAG351800.028.9522316-17
AGTACTT390550.028.10181420-21
Produced by FastQC (version 0.11.5)