FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15407094
Sequences flagged as poor quality0
Sequence length110
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1438140.933427160241899No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG613180.39798549940696154No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT222480.14440101423409243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA287750.065.628669
GGTATCA295750.063.6738858
TGGTATC297350.063.2709967
AAGCAGT303400.062.4635431
AGCAGTG308800.061.3371852
GTGGTAT310800.060.8601386
AGTGGTA331000.057.1552245
CAGTGGT334900.056.3771554
GCAGTGG478000.040.0514143
ATCAACG266750.038.5293910-11
CAACGCA287800.035.7954612-13
ACGCAGA295450.034.83511414-15
TCAACGC275400.034.28203612-13
AGAGTAC299750.034.1526118-19
AACGCAG300150.031.54334814-15
TATCAAC306000.031.01281410-11
GCAGAGT338250.030.48890116-17
GTACTTT319300.029.3607822-23
CGCAGAG322750.029.31050516-17
AGTACTT378100.026.9911720-21
Produced by FastQC (version 0.11.5)