FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15125885
Sequences flagged as poor quality0
Sequence length110
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1138030.7523725058070982No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG523960.34639956604192085No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT171150.1131504040920581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA248400.066.1425559
GGTATCA257800.063.4241188
TGGTATC265550.061.6253137
AGCAGTG264800.061.4642072
GTGGTAT280000.058.6439976
AAGCAGT279550.058.499991
CAGTGGT294950.055.415284
AGTGGTA296600.054.9166225
GCAGTGG409550.040.326173
ATCAACG232650.037.7382910-11
CAACGCA243100.036.18471512-13
TCAACGC235150.034.96431412-13
ACGCAGA260450.033.83611314-15
AGAGTAC259100.033.73111718-19
AACGCAG262650.031.46369714-15
TATCAAC265900.031.06369410-11
GCAGAGT301900.029.1388316-17
GTACTTT280950.029.07496322-23
CGCAGAG286450.028.72241216-17
GAGTACT295550.027.63289620-21
Produced by FastQC (version 0.11.5)