FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13325405
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC53150.018.161531
CAATGAA50600.017.5084198
ATGAATG53750.017.43123210
TGAGCCG38500.017.3297961
TTGACTT61800.017.2381041
GAAATGA59650.017.205421
TGCTACC52950.017.1911051
TTTACTG63050.017.0867311
CGGACAC45650.017.0258431
GGGACCT36050.016.9814411
ATGCGAT34200.016.905581
GCGCGAT28000.016.897851
TTGTAGG38350.016.849831
CAATGGA49400.016.8406988
AGGACGG33550.016.83925610
TGCCAAA44000.016.8231531
TGCGTGG51600.016.8072931
TTGGAAC37700.016.7423481
CGTTGGG46250.016.6751981
GGCTCGA37100.016.6356431
Produced by FastQC (version 0.11.5)