FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16899410
Sequences flagged as poor quality0
Sequence length110
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1741251.03036141498431No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG884170.5231957802077114No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT290050.17163321086357453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA340700.067.221879
GGTATCA346600.065.948598
AGCAGTG348900.065.200882
AAGCAGT352500.064.544581
TGGTATC355400.064.247447
GTGGTAT372800.061.2970926
AGTGGTA382750.059.703575
CAGTGGT394250.057.8999184
GCAGTGG530600.043.4051133
ATCAACG320350.038.66226610-11
CAACGCA341850.036.2225512-13
ACGCAGA351000.035.2597714-15
AGAGTAC354850.034.84924318-19
TCAACGC329550.034.65502512-13
AACGCAG355700.032.20571514-15
TATCAAC358850.031.99310510-11
GCAGAGT401300.030.96444716-17
CGCAGAG381050.030.03011916-17
GTACTTT379100.029.74494722-23
AGTACTT423000.029.07904420-21
Produced by FastQC (version 0.11.5)