FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15819794
Sequences flagged as poor quality0
Sequence length110
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1406560.8891139796131353No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG528710.3342078917083244No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT219280.1386111601706065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA293350.066.305849
GGTATCA298800.064.881698
AGCAGTG307000.063.1569942
TGGTATC308300.062.8080067
AAGCAGT315250.061.717911
GTGGTAT329250.058.9018336
CAGTGGT340350.057.002734
AGTGGTA341350.056.808885
GCAGTGG461700.042.3678743
ATCAACG275750.038.28827710-11
CAACGCA292350.036.0732312-13
ACGCAGA302600.034.8448814-15
TCAACGC282550.034.49010512-13
AGAGTAC304550.034.485618-19
AACGCAG307050.031.76544814-15
TATCAAC310800.031.64489610-11
GCAGAGT340600.030.9273616-17
CGCAGAG331050.029.51810516-17
GTACTTT324800.029.40840522-23
AGTACTT372600.027.95523520-21
Produced by FastQC (version 0.11.5)