FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15529588
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGC57800.017.5383781
CGGACTG52550.017.4261741
ATGCGAT48000.017.4118271
CAATGGA60900.017.3999068
GGATTAC57650.017.271871
TGCTACC52000.017.187531
CTTAGGA52400.017.1326431
CACACAA58600.017.1283451
TGGCTGG61000.017.0444431
GGGCATC51200.017.0265181
AGTGAAC49400.016.9175598
ACTGAAC43850.016.8938221
GACTAAC48100.016.8768041
GTCCGAA49350.016.8334148
AGATTGC51850.016.832351
TAAACCG53850.016.7270131
GGAACTT53350.016.7151281
CGACCTT57300.016.7143761
ATAACGC59850.016.687091
TGCGGGT41950.016.6580641
Produced by FastQC (version 0.11.5)