FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15529588
Sequences flagged as poor quality0
Sequence length110
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1705131.0979879182886243No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG831190.5352299107999516No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT266400.17154350778655558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA318400.069.2039
GGTATCA323950.067.961588
TGGTATC330400.066.7488867
AAGCAGT333900.066.5810851
AGCAGTG342200.064.87932
GTGGTAT347200.063.882866
AGTGGTA362850.060.8989645
CAGTGGT368150.060.0414
GCAGTGG509750.043.8032573
ATCAACG303050.039.12101410-11
CAACGCA321250.037.0639912-13
ACGCAGA331950.035.9744814-15
TCAACGC309950.035.66351312-13
AGAGTAC334350.035.52237718-19
AACGCAG336450.032.9967314-15
TATCAAC338500.032.69674710-11
GCAGAGT375200.031.93232216-17
CGCAGAG358200.030.9938616-17
GTACTTT357300.030.7521122-23
AGTACTT420250.028.15478320-21
Produced by FastQC (version 0.11.5)