FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16051743
Sequences flagged as poor quality0
Sequence length110
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1830341.1402749221688884No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG812840.5063873748788527No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT314000.1956173856010528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG349700.067.778152
AAGCAGT359650.066.0857851
GTATCAA362150.065.953489
GGTATCA367150.065.090688
TGGTATC372900.064.063077
GTGGTAT391800.061.065356
AGTGGTA406150.058.716045
CAGTGGT407000.058.4546434
GCAGTGG536150.044.794013
ATCAACG343050.038.0078210-11
CAACGCA362500.035.9322512-13
AGAGTAC372000.035.0319418-19
ACGCAGA373000.034.97217614-15
TCAACGC350850.034.09398712-13
TATCAAC378100.031.78210810-11
AACGCAG377250.031.77250114-15
GCAGAGT412750.031.7371316-17
CGCAGAG397600.030.20579516-17
GTACTTT394200.030.15602722-23
AGTACTT441650.029.36392220-21
Produced by FastQC (version 0.11.5)