FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14509842
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGGTCTTGATTAATGAAAACATTC332560.22919615527171142No Hit
GTCTCGTTCGTTATCGGAATTAACCA194350.1339435674075569No Hit
CGTTCGTTATCGGAATTAACCAGACA170780.11769942084827664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTACG975650.019.7774241
CTACGGT947100.019.6564943
TACGGTA946200.019.6561684
GGACAGA388500.019.6512761
GGTAGCC948000.019.6284087
CGGTAGC948050.019.6220326
ACTTGTG499350.019.6132812
TCTACGG951250.019.609772
AGCCTCG946900.019.59874310
ACGGTAG949450.019.5962585
TACTTGT511000.019.5909861
GGACGTC263000.019.5742421
GTAGCCT951650.019.562388
TAGCCTC951800.019.5319129
TTGTGTC499350.019.5290374
CTAACTT435700.019.5081311
CTTGTGT500900.019.4925633
TCCAGAA262350.019.4582568
ACCGAGA239100.019.43543610
TCTAGCC500900.019.4264839
Produced by FastQC (version 0.11.5)