FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14681635
Sequences flagged as poor quality0
Sequence length110
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1387310.9449288175329246No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG845150.5756511451210986No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT217070.1478513803128875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA271550.067.8928769
GGTATCA280350.065.718858
TGGTATC286900.064.064337
AGCAGTG287200.063.8579372
AAGCAGT294650.062.2913361
GTGGTAT304700.060.5854726
AGTGGTA312800.058.7555585
CAGTGGT317350.057.9358254
GCAGTGG432150.042.8873183
ATCAACG256900.038.729710-11
CAACGCA269050.037.13173312-13
ACGCAGA279250.035.6206714-15
TCAACGC260700.035.50829712-13
AGAGTAC282500.035.1560618-19
AACGCAG284200.032.57539714-15
TATCAAC290150.031.99757210-11
GCAGAGT326300.030.65237816-17
CGCAGAG306150.030.24890516-17
GTACTTT302950.030.11495222-23
AGTACTT341900.028.97042820-21
Produced by FastQC (version 0.11.5)